Alafia River Corridor Nature Preserve is a 4,700 acre area of protected lands in Hillsborough County, Florida. There are two trailheads for the preserves's hiking trails, one located at the north boundary of the Pinecrest Sports Complex on Hwy. 39 approximately one mile north of Hwy. 640 and one located on Hwy. 39 just north of Jameson Rd.